Altitudinal gradients in Magellanic sub-Antarctic lagoons: the effect of elevation on freshwater macroinvertebrate diversity and distribution Author Rendoll Cárcamo, Javier Wankara Sub-Antarctic and Antarctic Freshwater Ecosystems Laboratory, Universidad de Magallanes, Puerto Williams, Magallanes, Chile & Subantarctic Biocultural Conservation Program, Omora Ethnobotanical Park, Universidad de Magallanes, Puerto Williams, Magallanes, Chile & Institute of Ecology and Biodiversity, Universidad de Chile, Santiago, Chile javier.rendoll@gmail.com Author Contador, Tamara Wankara Sub-Antarctic and Antarctic Freshwater Ecosystems Laboratory, Universidad de Magallanes, Puerto Williams, Magallanes, Chile & Subantarctic Biocultural Conservation Program, Omora Ethnobotanical Park, Universidad de Magallanes, Puerto Williams, Magallanes, Chile & Institute of Ecology and Biodiversity, Universidad de Chile, Santiago, Chile & Núcleo Milenio de Salmónidos Invasores, INVASAL, Iniciativa Científica Milenio, ICM, Núcleo Científico Milenio, Concepción, Chile Author Gañán, Melisa Wankara Sub-Antarctic and Antarctic Freshwater Ecosystems Laboratory, Universidad de Magallanes, Puerto Williams, Magallanes, Chile Author Pérez Troncoso, Carolina Wankara Sub-Antarctic and Antarctic Freshwater Ecosystems Laboratory, Universidad de Magallanes, Puerto Williams, Magallanes, Chile & Subantarctic Biocultural Conservation Program, Omora Ethnobotanical Park, Universidad de Magallanes, Puerto Williams, Magallanes, Chile & Institute of Ecology and Biodiversity, Universidad de Chile, Santiago, Chile & Núcleo Milenio de Salmónidos Invasores, INVASAL, Iniciativa Científica Milenio, ICM, Núcleo Científico Milenio, Concepción, Chile Author Maldonado Márquez, Alan Wankara Sub-Antarctic and Antarctic Freshwater Ecosystems Laboratory, Universidad de Magallanes, Puerto Williams, Magallanes, Chile & Subantarctic Biocultural Conservation Program, Omora Ethnobotanical Park, Universidad de Magallanes, Puerto Williams, Magallanes, Chile & Institute of Ecology and Biodiversity, Universidad de Chile, Santiago, Chile & Núcleo Milenio de Salmónidos Invasores, INVASAL, Iniciativa Científica Milenio, ICM, Núcleo Científico Milenio, Concepción, Chile Author Convey, Peter British Antarctic Survey, NERC, Cambridge, United Kingdom Author Kennedy, James Wankara Sub-Antarctic and Antarctic Freshwater Ecosystems Laboratory, Universidad de Magallanes, Puerto Williams, Magallanes, Chile & Subantarctic Biocultural Conservation Program, Omora Ethnobotanical Park, Universidad de Magallanes, Puerto Williams, Magallanes, Chile & Department of Biological Sciences, University of North Texas, Denton, United States of America Author Rozzi, Ricardo Subantarctic Biocultural Conservation Program, Omora Ethnobotanical Park, Universidad de Magallanes, Puerto Williams, Magallanes, Chile & Institute of Ecology and Biodiversity, Universidad de Chile, Santiago, Chile & Department of Philosophy and Religion, University of North Texas, Denton, United States of America text PeerJ 2019 2019-07-29 7 1 18 https://doi.org/10.7717/peerj.7128 journal article 10.7717/peerj.7128 2167-8359 PMC6673463 31392086 14841445

Table 1 Study sites. Physical description of the four sampled lagoons along the elevation gradient of the Róbalo river watersed, Navarino Island, Chile.

Site	Name	Georeference	Elevation (m.a.s.l)	Water inflow and/or outflow	Adjacent vegetation
I	Castor lagoon	54 ◦ 56 , 19 ,, S/67 ◦ 38 , 15 ,, W	20	Diffuse	Evergreen forests and Sphagnum peatlands
II	Róbalo lake	54 ◦ 58 , 12 ,, S/67 ◦ 40 , 29 ,, W	250	South located inflow, outflow at North-East	Mixed deciduous and evergreen forests and scrublands
III	El Salto lagoon	54 ◦ 59 , 26 ,, S/67 ◦ 40 , 56 ,, W	480	South located inflow, outflow at North	Scrub forests, mosses and herbs
IV	Bandera lagoon	54 ◦ 58 , 26 ,, S/67 ◦ 38 , 41 ,, W	700	South-East located inflow, outflow at West	Mosses, lichens and cushion plants