Multigenetic characterization of ‘ Candidatus Xenohaliotis californiensis’ Author Cicala, Francesco Author Moore, James D. Author Cáceres-Martínez, Jorge Author Del Río-Portilla, Miguel A. Author Hernández-Rodríguez, Mónica Author Vásquez-Yeomans, Rebeca Author Rocha-Olivares, Axayácatl text International Journal of Systematic and Evolutionary Microbiology 2017 2017-01-01 67 1 42 49 http://dx.doi.org/10.1099/ijsem.0.001563 journal article 10.1099/ijsem.0.001563 1466-5034 6048375

Table 1. Haplotype-specific primers for Ca. Xc used in control tests

Locus	ID primers	Sequence (5 ′– 3 ′ )	Length Annealing temperature ( Ǫ C)		Expected fragment size (bp)
16S rRNA	ss16S.F	GCCTCAGTTTGGCTGGGTTCTTCA	24	66	426
	ss16S.R	GAATTGCCACTTTAAAGTATGGACGG	26
16S rRNA	ss16SV1.F	GCGGTAGGCTTAATACATGCAAGTTG	26	63	1412
	ss16S2.R	GTTGTCGCCCCCACTGTGAGTGGTTATC	28
23S rRNA	23S.s1F	CAGTTCGGTTTCTATCCTCCGCAA	24	66	376
	5S.s1R	GGGTGTTTCATCTCCCCTATCAAT	24
virD4	VirD4.s1F	CTCAGGGTCATGCTGTGTATTGC	23	66	289
	VirD4.s2R	CAACACAACTGCCAAATTGTAGGT	24
virB11	VirB11.ss2F	GCTCAATCTACTGAGCAGAAAGTTAG	26	60	508
	VirB11.ss1R	CGCAAGCAGGCTTCTATCAATTCTTG	26
ftsZ	ftsZ.ss1F	CCTGTAATTGCAAGAATTTCTCGTG	25	61	300
	ftsZ.ss1R	CTCTACCTTTGCCTTGCATAACAGAT	26