Ultrastructure of somatic embryo development and plant propagation for Lachenalia montana Author Baskaran, P. Author Van Staden, J. text South African Journal of Botany 2017 2017-03-31 109 10 269 274 http://dx.doi.org/10.1016/j.sajb.2017.01.006 journal article 10.1016/j.sajb.2017.01.006 1727-9321 14036627

Table 2 Enhancement of somatic embryos and germination frequency in cell suspension culture from friable embryogenic callus (FEC) of L. montana.

Sucrose (g L − 1) + PGR (μM) in solid MS medium	PGR (μM) in liquid MS medium	Liquid MS medium (MSL)	Number of SEs/SCV developmental stage		Germination (%)
PGR = Plant growth regulator. MSL = Liquid MS medium.SEs = Somatic embryos.SCV = Settled cell volume.*Different stages of embryo = pear-shaped, early torpedo-shaped, torpedoshaped and cotyledonary-stage embryos. Values with the means derived from 5 replicate (each 500 mg FEC) with 1000 μL SCV of embryogenic suspension cells per replicate. Mean values followed by same letters in each column are not significantly different according to the Duncan's multiple range test at 5% level.
6 weeks of culture	4 weeks of culture	2 weeks of culture	Globular	Different stages of embryo*
35 + 10 picloram + 2 BA	1 picloram	MSL	10.0 e	6.4 e	0
	1 2,4-D	MSL	14.4 cd	9.0 cd	0
	1 2,4-D + 0.5 BA	MSL	15.0 cd	9.8 cd	7.2 e
	1 2,4-D + 0.5 m TR	MSL	7.8 f	6.0 e	8.6 de
	1 2,4-D + 0.5 TDZ	MSL	18.0 b	12.4 b	14.0 ab
35 + 10 2,4-D + 2 TDZ	1 picloram	MSL	6.8 fg	3.0 f	0
	1 2,4-D	MSL	16.0 cd	6.8 e	0
	1 2,4-D + 0.5 BA	MSL	18.4 b	10.0 c	11.4 c
	1 2,4-D + 0.5 m TR	MSL	10.2 e	6.8 e	14.3 ab
	1 2,4-D + 0.5 TDZ	MSL	21.4 a	14.6 a	15.2 a