Microsatellite development in the freshwater red alga Batrachospermum gelatinosum (L.) De Candolle (Batrachospermales, Rhodophyta)
Author
Crowell, Roseanna M.
Department of Environmental and Plant Biology, Ohio University, Athens, OH, 45701 (United States)
Author
Shainker-Connelly, Sarah J.
Department of Biology, University of Alabama at Birmingham, Birmingham, AL, 35294 (United States)
Author
Vis, Morgan L.
Department of Environmental and Plant Biology, Ohio University, Athens, OH, 45701 (United States)
Author
Krueger-Hadfield, Stacy A.
Department of Biology, University of Alabama at Birmingham, Birmingham, AL, 35294 (United States) and Virginia Institute of Marine Science Eastern Shore Laboratory, Wachapreague, VA, 23480 (United States) sakh @ vims. edu (corresponding author)
sakh@vims.edu
text
Cryptogamie, Algologie
2024
2024-05-15
45
5
53
62
https://sciencepress.mnhn.fr/sites/default/files/articles/pdf/algologie2024v45a5.pdf
journal article
10.5252/cryptogamie-algologie2024v45a5
1776-0984
11203720
TABLE 2. — Microsatellite locus information for Batrachospermum gelatinosum (L.) De Candolle. Locus name, repeat motif, expected size, oligo sequences, agarose gel amplification profile,fluorochrome used on the forward oligo,and fragment analysis (FA) amplification profile. Note: fluorochrome and FA profile columns are only for loci tested on the capillary sequencer. Diallelic 1, we consistently observed two alleles when only one should be present in a haploid gametophyte. | | Exp. | | | | FA | Allele size | |
|---|
| Locus | Motif | size | Oligo sequences | Gel profile | Fluorochrome | profile | range | Total alleles |
|---|
| Bgel_021 | AGG | 144 bp | F: GTTTCGAAGCTCAGTGTCCG | Single | 6-FAM | Single | 138-141 | 2 |
|---|
| | | R: GGAATTCTCGACGCACTTGG | | | | | |
|---|
| Bgel_052 | AT | 312 bp | F: GGGTCAATGCAAGTGGATGG | Single | VIC | Single | 310-312 | 2 |
|---|
| | | R: AGACCTTGGAAGCTACGACG | | | | | |
|---|
| Bgel_053 | AG | 327 bp | F: GCTGTCATGTCGCCAGAATG | Single | VIC | Single | 298-328 | 6 |
|---|
| | | R: GGAAGATGCACCTTTGGACG | | | | | |
|---|
| Bgel_056 | AG | 273 bp | F: CTTGCTCACGACTTTGGACC | Single | VIC | Single | 272-284 | 5 |
|---|
| | | R: CGGAGTGAAACGAAACGAGG | | | | | |
|---|
| Bgel_057 | AGC | 396 bp | F: GCTGAATGAGGTGATGTGGC | Single | PET | Single | 382-412 | 7 |
|---|
| | | R: TGCACGTGGTTCTTGACAAG | | | | | |
|---|
| Bgel_059 | AGG | 272 bp | F: TTTGAGTACCACCACCCGTC | Single | VIC | Single | 252-258 | 2 |
|---|
| | | R: GGGAAGTAGGCGTAGAAGGG | | | | | |
|---|
| Bgel_067 | AG | 191 bp | F: AGGCCAACATGCAGCAATAG | Single | 6-FAM | Single | 178-182 | 3 |
|---|
| | | R: CAAGTTGCTTTGTTCGCTGC | | | | | |
|---|
| Bgel_070 | AG | 130 bp | F: TGGAGGCTAACGACATGGAC | Single | 6-FAM | Single | 120-140 | 7 |
|---|
| | | R: CCGCACAAAGTAGTCGATCG | | | | | |
|---|
| Bgel_071 | AG | 226 bp | F: TTATCCACTCCCGGTCTTGC | Single | NED | Single | 215-299 | 14 |
|---|
| | | R: GTTTGAAGCGTGGGAAGAGG | | | | | |
|---|
| Bgel_073 | ACG | 294 bp | F: TCGACTTTGCAAACTCCAGC | Single | VIC | Single | 272-308 | 6 |
|---|
| | | R: GGTACGTGTGGACAAACGAC | | | | | |
|---|
| Bgel_035 | ACG | 424 bp | F: GTTGGCGGAAATGGAGTGAG | Single | PET | Diallelic1 | – | – |
|---|
| | | R: CTTCGACATCATGCTGAGCG | | | | | |
|---|
| Bgel_048 | AG | 348 bp | F: AACTTGGCCACGCATTCATC | Single | PET | Diallelic1; | – | – |
|---|
| | | R: CAATGGTCATCTGCCGTGTC | | | poor amp. | |
|---|
| Bgel_006 | ATC | 200 bp | F: CTCGTTCAAAGCTAGGCGTG | Single | NED | Multiple | – | – |
|---|
| | | R: TAAACAGGCCCTATGTCCGG | | | | | |
|---|
| Bgel_011 | AGG | 261 bp | F: CTGCTTCGACACCAACGTAC | Single | NED | Multiple | – | – |
|---|
| | | R: TCTCTGCCTCTCCATTCACG | | | | | |
|---|
| Bgel_031 | AGCC | 372 bp | F: CTCTGGTGCGCTGTTTATCG | Single | PET | Multiple | – | – |
|---|
| | | R: ACCAAACGGAAACAGCTGAC | | | | | |
|---|
| Bgel_041 | AAC | 211 bp | F: TGAAGGCGTTGTTGGGAAAC | Single | NED | Multiple; | – | – |
|---|
| | | R: GGTGGATTCAAGCGCCTATC | | | poor amp. | |
|---|
| Bgel_047 | AAT | 183 bp | F: TAAGGTCGCTTCTCCACCAC | Single | 6-FAM | Multiple | – | – |
|---|
| | | R: ATTCAAGCCTTCGAAGCTGC | | | | | |
|---|
| Bgel_058 | AG | 367 bp | F: CCGTTTCTGCAGTCGTCATC | Single | PET | Multiple | – | – |
|---|
| | | R: CCTGAAGCTGCTGGAAATCG | | | | | |
|---|
| Bgel_001 | AAC | 292 bp | F: GTTGACCGGTGTTCAAGTCG | Multiple | – | – | – | – |
|---|
| | | R: GATTCGTCGCTTCGGAATCC | | | | | |
|---|
| Bgel_002 | AAC | 166 bp | F: GCGGACACAAAGCAGTAGAC | Multiple | – | – | – | – |
|---|
| | | R: ACAACAACGACAATGGACCG | | | | | |
|---|
| Bgel_003 | AGC | 241 bp | F: ACAGGAGTATGCAGAACCGG | Multiple | – | – | – | – |
|---|
| | | R: GAAAGCTGCACTCCACCATC | | | | | |
|---|
| Bgel_004 | AGG | 255 bp | F: AGTACACGAGCCACCATCTC | Multiple | – | – | – | – |
|---|
| | | R: TGAGAGGAAGCAGCAGTCAC | | | | | |
|---|
| Bgel_005 | AAC | 231 bp | F: GTGGAGCCAACACGTTACG | Multiple | – | – | – | – |
|---|
| | | R: TCCTGGAGTGTTACTGGCTG | | | | | |
|---|
| Bgel_007 | AAG | 141 bp | F: GGTGCTGGTTGATTGATGGG | Multiple | – | – | – | – |
|---|
| | | R: TGAGAACGAGGAGGCCAATC | | | | | |
|---|
| Bgel_008 | AGC | 308 bp | F: TTCGGTTCCGGTTCTACTCC | Multiple | – | – | – | – |
|---|
| | | R: GTCTTCCGTCTTTGCCATCG | | | | | |
|---|
| Bgel_009 | ATC | 273 bp | F: GCGTAATGGTGGTGGTCAAG | Multiple | – | – | – | – |
|---|
| | | R: ACCACTGGACGAGATGACTG | | | | | |
|---|
| Bgel_010 | AG | 277 bp | F: AGGCAGTTATCTTCCCGACC | Multiple | – | – | – | – |
|---|
| | | R: CACCGGATACTGACGTTTGC | | | | | |
|---|
| Bgel_012 | AGC | 346 bp | F: ACCACCTAGTTCTGCACCTC | Multiple | – | – | – | – |
|---|
| | | R: GACGATGCATGCGAGAGATG | | | | | |
|---|
| Bgel_013 | AGC | 302 bp | F: TGAAGGAGGCAGAGATGAGC | Multiple | – | – | – | – |
|---|
| | | R: CGTTCATCACTCGCGAAGAC | | | | | |
|---|
| Bgel_014 | AGC | 130 bp | F: GCAATATGAGGCAGCAAGGG | Multiple | – | – | – | – |
|---|
| | | R: TCTCAGCACGACACACTACC | | | | | |
|---|
| Bgel_015 | ATC | 167 bp | F: AGTGGATTGATTGTGCTGCG | Multiple | – | – | – | – |
|---|
| | | R: CCATCTTCGGTGCGTCTTTC | | | | | |
|---|
| Bgel_016 | AG | 213 bp | F: ACGGCGATGATTTGTTTCCC | Multiple | – | – | – | – |
|---|
| | | R: AACAGTACTCCGCTCTCGTC | | | | | |
|---|
| Locus | Motif | size | Oligo sequences | Gel profile | Fluorochrome | profile | range | Total alleles |
|---|
| Bgel_017 | AAG | 176 bp | F: TCCTTTCTCCTCCTCGCAAC | No amp. | – | – | – | – |
|---|
| | | R: GACCTGGACGTTTGAATCGC | | | | | |
|---|
| Bgel_018 | AAG | 290 bp | F: GCACAGATACATTCGGCGTC | Multiple | – | – | – | – |
|---|
| | | R: TCCCATCGAACATCCACCTC | | | | | |
|---|
| Bgel_019 | AT | 241 bp | F: GTACTTATGTGCGGCCTTGG | Multiple | – | – | – | – |
|---|
| | | R: CAGTCCCGGTCTATGTAGGC | | | | | |
|---|
| Bgel_020 | AG | 315 bp | F: TAGAATGAGACGGGCGATCG | Multiple | – | – | – | – |
|---|
| | | R: CCGCTTTGAGTCCGTAAACC | | | | | |
|---|
| Bgel_022 | AC | 298 bp | F: GCCATCCTCTTGCCACATTC | No amp. | – | – | – | – |
|---|
| | | R: GTTTGGGTTCCGTCTGTCAG | | | | | |
|---|
| Bgel_023 | ACT | 161 bp | F: TGTCGACCATAAGCTCCGAG | No amp. | – | – | – | – |
|---|
| | | R: GTCACCTGGGCAAGCATTAC | | | | | |
|---|
| Bgel_024 | AAC | 236 bp | F: TTGCGCAGATTCACGAACTG | Multiple | – | – | – | – |
|---|
| | | R: AGGTGATAAGAGGCGGTGAC | | | | | |
|---|
| Bgel_025 | ACT | 244 bp | F: TGAGTGATTGCGGCCATTTC | No amp. | – | – | – | – |
|---|
| | | R: AGTGGCACCTCGATATACCG | | | | | |
|---|
| Bgel_026 | AGC | 282 bp | F: TCTGATGGTAGGGTTGCTGG | Multiple | – | – | – | – |
|---|
| | | R: AGAGGGCTGTAGTGAATCGG | | | | | |
|---|
| Bgel_027 | AGG | 190 bp | F: ATCGGTCAAGAGTTGCATGC | Multiple | – | – | – | – |
|---|
| | | R: ACGTCTCTATTCCATCGCCC | | | | | |
|---|
| Bgel_028 | AC | 389 bp | F: ATTGCTCCGTCATTGGCATG | No amp. | – | – | – | – |
|---|
| | | R: ACTCACACCACACTCCGTAG | | | | | |
|---|
| Bgel_029 | ACTC | 330 bp | F: TCGCGCTCATTTCCAAACTC | Multiple | – | – | – | – |
|---|
| | | R: TCAGTCGATCAAGGAGCTGG | | | | | |
|---|
| Bgel_030 | AAT | 280 bp | F: GCTGCGTCACTCTTCTCATG | No amp. | – | – | – | – |
|---|
| | | R: TTTCCTCTTGTTGTGCTCGC | | | | | |
|---|
| Bgel_032 | ATC | 226 bp | F: GATTCCAAATACCACCGGCG | Multiple | – | – | – | – |
|---|
| | | R: ATCGCCTGGGATGATCGATC | | | | | |
|---|
| Bgel_033 | ACGG | 159 bp | F: CCTGCACTTGTGACGATTCC | No amp. | – | – | – | – |
|---|
| | | R: GGACGCTTCGAAGGAACATC | | | | | |
|---|
| Bgel_034 | ATC | 281 bp | F: CGTCGTCGTCAATGTTCCTG | Multiple | – | – | – | – |
|---|
| | | R: CCTTGCTGTGGAACTTGGTG | | | | | |
|---|
| Bgel_036 | AC | 179 bp | F: TCGTCCTGGTCCATGCTAAG | No amp. | – | – | – | – |
|---|
| | | R: CCTGCCCGTTTGCTTATGAG | | | | | |
|---|
| Bgel_037 | AAC | 179 bp | F: CCTCCCAACGAAACATCAGC | No amp. | – | – | – | – |
|---|
| | | R: ATTACGAGTGTCACCGGGAG | | | | | |
|---|
| Bgel_038 | AGAT | 159 bp | F: CTATTTCGATTCGTCGCGGG | Multiple | – | – | – | – |
|---|
| | | R: AAGACAGAACCTCCGCTCAG | | | | | |
|---|
| Bgel_039 | AAT | 148 bp | F: CCACTTCGGTTTCAGGAAGC | No amp. | – | – | – | – |
|---|
| | | R: CGGTCAGAATCATCACGTGC | | | | | |
|---|
| Bgel_040 | ATC | 140 bp | F: TCCTCTCCATTCAGCAGTCG | Multiple | – | – | – | – |
|---|
| | | R: CAAGAGAAGCTGAAGACGCG | | | | | |
|---|
| Bgel_042 | AC | 418 bp | F: TGGACATACTCGCTCCACAG | No amp. | – | – | – | – |
|---|
| | | R: GCAGCGCTTAGAGTGTGAAG | | | | | |
|---|
| Bgel_043 | AAT | 275 bp | F: TCCAACTCCTCAACGACCTG | Multiple | – | – | – | – |
|---|
| | | R: TTGGAGCAGAATTCGTTCGC | | | | | |
|---|
| Bgel_044 | AAC | 333 bp | F: AGCCAATACCAACCCTCGAG | Multiple | – | – | – | – |
|---|
| | | R: TGCATCATTCAACTCGCCAC | | | | | |
|---|
| Bgel_045 | AAC | 239 bp | F: ACCGAACAAGCACTTCAACC | Multiple | – | – | – | – |
|---|
| | | R: ATGACGTCGTTGGCAAGAAC | | | | | |
|---|
| Bgel_046 | AAC | 173 bp | F: CAGCAGCGAGTGGAAAGTAC | Multiple | – | – | – | – |
|---|
| | | R: GCTACACGAAGATGGGCAAC | | | | | |
|---|
| Bgel_049 | AATT | 395 bp | F: GTTCGACGGTCATCAGCATG | No amp. | – | – | – | – |
|---|
| | | R: TCTTCCAACCCTGCTCTGAC | | | | | |
|---|
| Bgel_050 | AGG | 251 bp | F: AAGTGGAGGAGGAATGGGTG | No amp. | – | – | – | – |
|---|
| | | R: ACCTCTGCCTCACTCATTCC | | | | | |
|---|
| Bgel_051 | AAT | 147 bp | F: CATCATATCCCTGCCCTCCC | Multiple | – | – | – | – |
|---|
| | | R: AATGCAGCCATGACTCGTTG | | | | | |
|---|
| Bgel_054 | AC | 199 bp | F: TGCGTTGTCTTCTTTGCTCC | No amp. | – | – | – | – |
|---|
| | | R: CATCCGCCACAGAAACCATG | | | | | |
|---|
| Bgel_055 | AC | 282 bp | F: TCAGAGGAATGGATGGACGC | Multiple | – | – | – | – |
|---|
| | | R: GATGTTCCGTGCAGATCAGC | | | | | |
|---|
| Bgel_060 | AAG | 226 bp | F: CTTTCTTCACCGACGCTGAC | No amp. | – | – | – | – |
|---|
| | | R: CCGGGCTCAGAAATTTCGTC | | | | | |
|---|
| Bgel_061 | AAAT | 137 bp | F: CAGATGATTCGAGCGATGCC | No amp. | – | – | – | – |
|---|
| | | R: CACGGGCATGACAAATCTCC | | | | | |
|---|
| Locus | Motif | size | Oligo sequences | Gel profile | Fluorochrome profile | range | Total alleles |
|---|
| Bgel_062 | AG | 228 bp | F: CGAACAACAGACATAGCGGG | No amp. | – | – | – | – |
|---|
| | | R: TCTTCCTGCGGCTGTAAGAG | | | | | |
|---|
| Bgel_063 | AT | 148 bp | F: GTTCACCTGTGGAAGCGAAG | Multiple | – | – | – | – |
|---|
| | | R: AGTAATCGTCTCGCCTGGTG | | | | | |
|---|
| Bgel_064 | AAG | 315 bp | F: GCTTTGACCGAGTTGTACCG | Multiple | – | – | – | – |
|---|
| | | R: CGATTAGGCGGTTGTGTTGG | | | | | |
|---|
| Bgel_065 | AAG | 226 bp | F: GTTGACGACGCTTCATCGAG | Multiple | – | – | – | – |
|---|
| | | R: TGGGACTTCGAGTGGTTGAG | | | | | |
|---|
| Bgel_066 | AAC | 157 bp | F: TACACGTGTGAGGAGGCTTC | Multiple | – | – | – | – |
|---|
| | | R: ATTCACATCATCACCAGCGC | | | | | |
|---|
| Bgel_068 | AGC | 139 bp | F: TGCTGGACTAGTGACAGTGG | Multiple | – | – | – | – |
|---|
| | | R: TGCCTCACTACTGTCACCAC | | | | | |
|---|
| Bgel_069 | AGC | 217 bp | F: TGGGAGAAGACGAGCTGATG | Multiple | – | – | – | – |
|---|
| | | R: GGCTCAGAATCATGCTGACG | | | | | |
|---|
| Bgel_072 | AAG | 390 bp | F: GCCATCTTCATACGTCGCTG | Multiple | – | – | – | – |
|---|
| | | R: ACTTTGATCAGGCTCTCGGG | | | | | |
|---|