Molecular phylogeny of the Orthurethra (Panpulmonata: Stylommatophora) Author Saadi, Ahmed J. Author Mordan, Peter B. Author Wade, Christopher M. text Zoological Journal of the Linnean Society 2021 2021-01-10 193 1126 1140 journal article 10.1093/zoolinnean/zlaa168 0024-4082 7531947

Table 2. Primers used for polymerase chain reaction (PCR) amplification

Fragments	Primers	Reference	Fragment
			Size (bp)
Primary PCR	LSU-1ii (sense): 5’-CTAGCTGCGAGAATTAATGT	Wade & Mordan (2000); Wade et al.	~4000
	GA-3’ [Labelled as Primer LSU-1 in Wade et al.	(2001, 2006)
	(2006)]
	LSU-12 (anti-sense): 5’-TTCTGACTTAGAGG	Fontanilla et al. (2017); Saadi &
	CGTTCAG-3’	Wade (2019)
A	LSU-1ii (sense):	Wade & Mordan (2000); Wade et al.	~900–1200
	5’-CTAGCTGCGAGAATTAATGTGA-3’ (Labelled as	(2001, 2006)
	Primer LSU-1 in Wade et al. (2006)]
Or
	LSU-1iii (sense): 5’-TGCGAGAATTAATGTGAA	Fontanilla et al. (2017); (Saadi et al.
	TTGC-3’	(2020)
	LSU-3ii (anti-sense): 5’-ACTTTCCCTCACGGTA	Wade & Mordan (2000); Wade et al.
	CTTG-3’ [Labelled as Primer LSU-3 in Wade et al.	(2001, 2006)
	(2006)]
Or
	LSU-3iii (anti-sense): 5’-ACGGTACTTGTCCGC	Fontanilla et al. (2017); Saadi et al.
	TATCG-3’	(2020)
B	LSU-2ii (sense): 5’-GGGTTGTTTGGGAATGCAGC-3’	Wade & Mordan (2000); Wade et al.	~580
	[Labelled as Primer LSU-2 in Wade et al. (2006)]	(2001, 2006)
	LSU-5ii (anti-sense): 5’-GTTAGACTCCTTGGTCC	Wade & Mordan (2000); Wade et al.
	GTG-3’ [Labelled as Primer LSU-5 in Wade et al.	(2001, 2006)
(2006)]
C	LSU-4ii (sense): 5’-GTCGGCATTCCACCCGACC-3’	Fontanilla et al. (2017); Saadi &	~700
		Wade (2019)
Or
	LSU-4iii (sense): 5’-CGGTGGCGAGTCTGTCGGC-3’	Saadi et al. (2020)
	LSU-7 (anti-sense): 5’- GCAGGTGAGTTGTTACA	Fontanilla et al. (2017); Saadi &
	CACTC-3’	Wade (2019)
	LSU-7i (anti-sense): 5’-GTTGTTACACACTCCTTAG	Fontanilla et al. (2017); Saadi et al.
	CGG-3’	(2020)
D	LSU-6i (sense): 5’-GTGCCAAACGCTGACGCTCA-3’	Fontanilla et al. (2017); Saadi &	~850
		Wade (2019)
	LSU-9i (anti-sense): 5’-ACCCAGTCCTCAGAGCC	Fontanilla et al. (2017); Saadi &
	AATC-3’	Wade (2019)
E	LSU-8ii (sense): 5’-GTGCACAGCCTCTAGTCGATA-3’	Fontanilla et al. (2017); Saadi &	~850
		Wade (2019)
	LSU-11ii (anti-sense): 5’-TCCTCCTGAGCTCGCC	Fontanilla et al. (2017); Saadi &
	TTAG-3’	Wade (2019)
F	LSU-10i (sense): 5’-GGCCGCGATCCGTCTGAAGA-3’	Fontanilla et al. (2017); Saadi &	~550
		Wade (2019)
	LSU-12i (anti-sense): 5’-GGCTTCTGACTTAGAGGC	Fontanilla et al. (2017); Saadi &
	GTT-3’	Wade (2019)