Molecular investigation and description of Iberozospeum n. gen., including the description of one new species (Eupulmonata, Ellobioidea, Carychiidae) Author Kneubühler, Jeannette Natural History Museum Bern, 3005 Bern, Switzerland & Institute of Ecology and Evolution, University of Bern, Author Jochum, Adrienne Natural History Museum Bern, 3005 Bern, Switzerland & Institute of Ecology and Evolution, University of Bern, & Senckenberg Research Institute and Natural History Museum, 60325 Frankfurt am Main, Germany Author Prieto, Carlos E. Departamento de Zoología Y Biología Celular Animal, Author Neubert, Eike Natural History Museum Bern, 3005 Bern, Switzerland & Institute of Ecology and Evolution, University of Bern, text Organisms Diversity & Evolution 2022 2021-11-05 22 1 61 92 http://dx.doi.org/10.1007/s13127-021-00517-9 journal article 300084 10.1007/s13127-021-00517-9 1738692d-b014-4e4c-a8ad-8fdfab35912b 1618-1077 12801053

Table 2 Primer designs used for PCR reactions

Gene	Primer	Sequence	Sequence length (bp)	Reference
COI	LCO1490	5’-GGTC AAC AAATCA TAAAGA TATT GG-3’	680	Folmer et al. (1994)
	HCO2198	5’-TAAACTTCAGGGT GACC AAAAAATC A-3’
16S	16S ar	5’-CGC CTG TTT ATC AAA AAC AT-3’	440	Simon et al. (1994)
	16S br	5’- CCG GTC TGA ACT CTG ATC AT -3’
H3	H3AD	5’-ATGGCTCG TA CCAA GCAGA CVGC-3’	380	Colgan et al. (1998)
	H3BD	5’-ATAT CCTTRGGCA TRATRG TGAC-3’
ITS2	ITS2ModA	5’-GCTTGCGGAG AATT AATGT GAA-3’	900	Bouaziz-Yahiatene et al. (2017)
	ITS2ModB	5’-GGTA CCTTGTT CGCTA TCGGA-3’