Hidden in the hills: phylogeny of the freshwater mussel genus Alasmidonta (Bivalvia: Unionidae) and description of a new species Author Whelan, Nathan V. Author Johnson, Nathan A. Author Ashantye Author Williams, S. Author Perkins, Michael A. Author Beaver, Caitlin E. Author Mays, Jason W. text Zoological Journal of the Linnean Society 2023 Zool. J. Linn. Soc. 2023-03-31 198 2 650 676 http://dx.doi.org/10.1093/zoolinnean/zlac106 journal article 54533 10.1093/zoolinnean/zlac106 7e3c51cd-a2ba-4789-8818-c4211f1ae300 0024-4082 8011159 EC0AE99D-08E2-40ED-A5E6-D03B322C65BC

Table 2. Collection information and GenBank accession numbers for mitochondrial genes ND1 and COI and nuclear genes ANT and ITS for individuals sequenced here. New species indicated in bold

Species	Laboratory code or	Location	Latitude	Longitude	ND1	COI	ANT	ITS
	museum catalogue		(° N)	(° W)
	number
Alasmidonta arcula	UF 438450.001	Altamaha River	31.6779	−81.8468	ON164855	ON157837	–	ON197362
Alasmidonta arcula	UF 439212.002	Altamaha River	31.7017	−81.8744	–	ON157838	ON164995	ON197363
Alasmidonta arcula	UF 439212.003	Altamaha River	31.7017	−81.8744	ON164856	ON157839	ON164996	ON197364
Alasmidonta arcula	UF 438439.004	Altamaha River	31.7949	−81.9996	–	ON157840	ON164997	ON197365
Alasmidonta arcula	UF 438439.005	Altamaha River	31.7949	−81.9996	–	ON157841	ON164998	ON197366
Alasmidonta arcula	UF 438352.006	Ocmulgee River	30.7934	−82.9793	ON164857	ON157842	ON165000	ON197367
Alasmidonta arcula	UF 438352.007	Ocmulgee River	30.7934	−82.9793	ON164858	ON157843	ON165001	ON197368
Alasmidonta arcula	UF 437994.009	Savannah River	32.4984	−81.2340	ON164859	ON157844	ON165007	ON197369
Alasmidonta arcula	UF 437999.010	Savannah River	32.8742	−81.4535	–	ON157845	ON165008	ON197370
Alasmidonta arcula	UF 437999.011	Savannah River	32.8742	−81.4535	ON164860	ON157846	ON165009	ON197371
Alasmidonta arcula	UF 437999.012	Savannah River	32.8742	−81.4535	ON164861	ON157847	ON165010	ON197372
Alasmidonta arcula	UF 437999.013	Savannah River	32.8742	−81.4535	ON164862	ON157848	ON165011	ON197373
Alasmidonta arcula	UF 437999.014	Savannah River	32.8742	−81.4535	ON164863	ON157849	ON165012	ON197374
Alasmidonta arcula	UF 437999.015	Savannah River	32.8742	−81.4535	ON164864	ON157850	ON165013	ON197375
Alasmidonta arcula	UF 438007.016	Savannah River	32.8407	−81.4257	ON164865	ON157851	ON165014	ON197376
Alasmidonta arcula	UF 438009.017	Savannah River	32.5182	−81.2426	ON164866	ON157852	ON165015	ON197377
Alasmidonta arcula	UF 439049.9032	Savannah River	32.4665	−81.1984	–	ON157853	ON165020	ON197378
Alasmidonta uaeharriensis	USNM 1675683	Barnes Creek	35.4782	−79.9584	ON164868	ON157855	ON164932	ON197380
Alasmidonta uaeharriensis	USNM 1675684	Barnes Creek	35.4782	−79.9584	ON164869	ON157856	ON164933	ON197381
Alasmidonta uaeharriensis	USNM 1675685	Barnes Creek	35.4344	−79.9999	–	ON157857	ON164934	ON197382
Alasmidonta uaeharriensis	USNM 1675686	Densons Creek	35.3927	−79.8778	ON164870	ON157858	ON164935	ON197383
Alasmidonta uaeharriensis	USNM 1675687	Densons Creek	35.3927	−79.8778	ON164871	ON157859	ON164936	ON197384
Alasmidonta uaeharriensis	Den_1	Densons Creek	35.3864	−78.8679	ON164904	ON157893	ON164967	ON197415
Alasmidonta uaeharriensis	Den_2	Densons Creek	35.3864	−78.8679	ON164905	ON157894	ON164968	ON197416
Alasmidonta uaeharriensis	Den_3	Densons Creek	35.3864	−78.8679	ON164906	ON157895	ON164969	ON197417
Alasmidonta uaeharriensis	Dumas_1_a	Dumas Creek	35.3935	−79.8972	ON164910	ON157897	ON164971	ON197419
Alasmidonta uaeharriensis	Dumas_2_a	Dumas Creek	35.3935	−79.8972	ON164911	ON157898	ON164972	ON197420
Alasmidonta uaeharriensis	FHR_1	Barnes Creek	35.4798	−79.9527	–	ON157899	–	–
Alasmidonta uaeharriensis	FHR_2	Barnes Creek	35.4798	−79.9527	ON164912	ON157900	ON164973	ON197421
Alasmidonta uaeharriensis	FHR_3	Barnes Creek	35.4798	−79.9527	ON164913	ON157901	ON164974	ON197422
Alasmidonta uaeharriensis	FHR_4	Barnes Creek	35.4798	−79.9527	ON164914	ON157902	ON164975	–
Alasmidonta uaeharriensis	Pee_Dee_1	Hannah’s Creek	35.5846	−79.9434	–	ON157909	ON164983	ON197429
Alasmidonta uaeharriensis	Pee_Dee_2	Tom’s Creek	35.6399	−79.9792	–	ON157910	ON164984	ON197430
Alasmidonta raveneliana	ALRA_FB1	Little River	35.2264	−82.6401	ON164875	ON157860	ON164942	–
(French Broad)
	museum catalogue		(° N)	(° W)
	number
Alasmidonta raveneliana	ALRA_FB2	Little River	35.2264	−82.6401	ON164876	ON157861	ON164943	–
(French Broad)
Alasmidonta raveneliana	ALRA_FB3	Little River	35.2264	−82.6401	ON164877	ON157862	ON164944	–
(French Broad)
Alasmidonta raveneliana	ALRA_FB4	Little River	35.2264	−82.6401	ON164878	ON157863	ON164945	–
(French Broad)
Alasmidonta raveneliana	ALRA_FB5	Little River	35.2264	−82.6401	ON164879	ON157864	ON164946	–
(French Broad)
Alasmidonta raveneliana	ALRA_LT1	Burningtown	32.2775	−83.4803	ON164880	ON157865	–	ON197388
(Little Tennessee)		Creek
Alasmidonta raveneliana	ALRA_LT2	Burningtown	32.2775	−83.4803	ON164881	ON157866	ON164947	ON197389
(Little Tennessee)		Creek
Alasmidonta raveneliana	ALRA_LT3	Burningtown	32.2775	−83.4803	ON164882	ON157867	ON164948	ON197390
(Little Tennessee)		Creek
Alasmidonta raveneliana	ALRA_LT4	Burningtown	32.2775	−83.4803	ON164883	ON157868	ON164949	ON197391
(Little Tennessee)		Creek
Alasmidonta raveneliana	ALRA_LT5	Burningtown	32.2775	−83.4803	ON164884	ON157869	ON164950	ON197392
(Little Tennessee)		Creek
Alasmidonta triangulata	AtriApa004	Apalachicola	30.1328	−85.1372	–	ON157870	–	–
		River
Alasmidonta triangulata	UF	Chipola River	30.1026	−85.1766	ON164885	ON157871	ON165025	ON197393
	439359.11408
Alasmidonta triangulata	UF	Chipola River	30.1026	−85.1766	ON164886	ON157872	ON165026	ON197394
	439359.11409
Alasmidonta triangulata	UF 438251.6276	Chipola River	30.4308	−85.1718	ON164887	ON157873	ON165019	–
Alasmidonta triangulata	UF 439089.9865	Chipola River	30.1028	−85.1764	–	–	ON165023	ON197395
Alasmidonta triangulata	UF 439089.9866	Chipola River	30.1028	−85.1764	ON164888	ON157874	ON165024	ON197396
Alasmidonta triangulata	UF	Chipola River	30.2262	−85.1931	ON164889	ON157875	ON165027	ON197397
439447.11852
Alasmidonta triangulata	UF 441077.001	Flint River	31.0052	−84.5226	ON164890	ON157876	ON165003	ON197398
Alasmidonta triangulata	UF 441077.002	Flint River	31.0052	−84.5226	MG199739	MG199612	ON165004	MG199866
Alasmidonta triangulata	UF 441077.003	Flint River	31.0052	−84.5226	ON164891	ON157877	ON165005	ON197399
Alasmidonta varicosa	Cacapon_1	Cacapon River	39.6164	−78.2810	ON164892	ON157878	ON164951	ON197400
Alasmidonta varicosa	Cacapon_2	Cacapon River	39.6164	−78.2810	ON164893	–	ON164952	ON197401
Alasmidonta varicosa	Cacapon_3	Cacapon River	39.6164	−78.2810	ON164894	ON157879	ON164953	ON197402
Alasmidonta varicosa	Cacapon_4	Cacapon River	39.6164	−78.2810	ON164895	ON157880	ON164954	ON197403
Alasmidonta varicosa	Cacapon_5	Cacapon River	39.6164	−78.2810	ON164896	–	ON164955	ON197404
	museum catalogue		(° N)	(° W)
number
Alasmidonta varicosa	Cat_1	Catawba River	35.6442	−82.1127	ON164897	ON157881	ON164956	ON197405
Alasmidonta varicosa	Chatt_1	Chatooga River	34.8660	−83.2341	–	ON157882	ON164957	ON197406
Alasmidonta varicosa	Chatt_2	Chatooga River	34.8660	−83.2341	–	ON157883	ON164958	ON197407
Alasmidonta varicosa	Chatt_3	Chatooga River	34.8481	−83.2341	–	ON157884	ON164959	ON197408
Alasmidonta varicosa	Chatt_4	Chatooga River	34.8481	−83.2341	–	ON157885	ON164960	ON197409
Alasmidonta varicosa	Chatt_5	Chatooga River	34.8660	−83.2341	ON164898	ON157886	ON164961	ON197410
Alasmidonta varicosa	Chatt_6	Chatooga River	34.8481	−83.2341	ON164899	ON157887	ON164962	ON197411
Alasmidonta varicosa	Don_1	Donnaha River	36.2218	−80.4342	ON164907	ON157896	ON164970	ON197418
Alasmidonta varicosa	Maryland_1	Potomac River	39.6025	−77.9078	ON164916	ON157903	ON164976	ON197423
Alasmidonta varicosa	Maryland_2	Potomac River	39.6025	−77.9078	ON164917	–	ON164977	ON197424
Alasmidonta varicosa	Mulberry_1	Mulberry Creek	35.9545	−81.6253	ON164918	ON157904	ON164978	ON197425
Alasmidonta varicosa	Mulberry_2	Mulberry Creek	35.9545	−81.6253	ON164919	ON157905	ON164979	ON197426
Alasmidonta varicosa	Mulberry_3	Mulberry Creek	35.9545	−81.6253	ON164920	ON157906	ON164980	–
Alasmidonta varicosa	Mulberry_4	Mulberry Creek	35.9545	−81.6253	ON164921	ON157907	ON164981	ON197427
Alasmidonta varicosa	Mulberry_5	Mulberry Creek	35.9545	−81.6253	ON164922	ON157908	ON164982	ON197428
Alasmidonta varicosa	Roar_1	Roaring River	36.3249	−81.0910	ON164923	ON157911	ON164985	ON197431
Alasmidonta varicosa	Turkey_1	Turkey Creek	29.8299	−82.1137	ON164925	ON157913	ON164987	ON197432
Alasmidonta varicosa	Turkey_3	Turkey Creek	31.8299	−82.1137	ON164927	ON157915	ON164989	ON197433
Alasmidonta varicosa	Turkey_4	Turkey Creek	32.8299	−82.1137	ON164928	ON157916	ON164990	ON197434
Alasmidonta varicosa	Turkey_5	Turkey Creek	33.8299	−82.1137	ON164929	ON157917	ON164991	ON197435
Alasmidonta varicosa	Wilson_1	Wilson’s Creek	35.8871	−81.7166	–	–	ON164993	ON197436
Alasmidonta varicosa	Wilson_2	Wilson’s Creek	35.8871	−81.7166	ON164931	ON157919	ON164994	ON197437
Alasmidonta varicosa (Cape Fear)	Deep_1	Deep River	35.4779	−79.5197	ON164900	ON157888	–	ON197412
Alasmidonta varicosa (Cape Fear)	Deep_2	Deep River	35.4779	−79.5197	ON164901	ON157889	ON164963	ON197413
Alasmidonta varicosa (Cape Fear)	Deep_3	Deep River	35.4779	−79.5197	ON164902	ON157890	ON164964	ON197414
Alasmidonta varicosa (Cape Fear)	Deep_4	Deep River	35.4779	−79.5197	ON164903	ON157891	ON164965	–
Alasmidonta varicosa (Cape Fear)	Deep_5	Deep River	35.4779	−79.5197	–	ON157892	ON164966	–
Alasmidonta varicosa (Cape Fear)	RR_2	Rock River	35.6545	−79.2394	ON164924	ON157912	ON164986	–
Alasmidonta viridis	Alas_S6	Mills River	35.38744	−82.5464	–	–	ON164937	ON197385
Alasmidonta viridis	Alas_S7	Mills River	35.38744	−82.5464	ON164873	MT985190	ON164938	ON197386
Alasmidonta viridis	Alas_S8	Mills River	35.38744	−82.5464	ON164874	–	ON164939	ON197387
Alasmidonta viridis	Alas_S9	Mills River	35.38744	−82.5464	–	–	ON164940	–
Alasmidonta viridis	Alas_S10	Mills River	35.38744	−82.5464	ON164872	–	ON164941	–
Lasmigona etowaensis	UF 438474.003	Youngs Mill	34.2579	−85.3014	MG199738	MG199611	ON165002	MG199865
		Creek