A new species Pseudoplagiostoma dipterocarpicola (Pseudoplagiostomataceae, Diaporthales) found in northern Thailand on members of the Dipterocarpaceae Author Tang, Xia 0000-0003-2705-604X Engineering and Research Center for Southwest Biopharmaceutical Resource of National Education Ministry of China, Guizhou University, Guiyang, 550025, Guizhou Province, People’s Republic of China & Center of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai, 57100, Thailand & School of science, Mae Fah Luang University, Chiang Rai, 57100, Thailand & bella 19580412 @ gmail. com; https: // orcid. org / 0000 - 0003 - 2705 - 604 X bella19580412@gmail.com Author Jayawardena, Ruvishika S. 0000-0001-7702-4885 Center of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai, 57100, Thailand & School of science, Mae Fah Luang University, Chiang Rai, 57100, Thailand & ruvi. jaya @ yahoo. com; https: // orcid. org / 0000 - 0001 - 7702 - 4885 ruvi.jaya@yahoo.com Author Stephenson, Steven L. 0000-0002-9207-8037 Department of Biological Sciences University of Arkansas Fayetteville AR 72701, USA & slsteph @ uark. edu; https: // orcid. org / 0000 - 0002 - 9207 - 8037 slsteph@uark.edu Author Kang, Ji-Chuan 0000-0002-6294-5793 Engineering and Research Center for Southwest Biopharmaceutical Resource of National Education Ministry of China, Guizhou University, Guiyang, 550025, Guizhou Province, People’s Republic of China & jckang @ gzu. edu. cn; https: // orcid. org / 0000 - 0002 - 6294 - 5793 jckang@gzu.edu.cn text Phytotaxa 2022 2022-04-20 543 4 233 243 journal article 55864 10.11646/phytotaxa.543.4.3 da817059-471c-4f31-bb76-383057d3a637 1179-3163 6479390

TABLE 2. Primers and PCR amplification conditions

Locus	Primers (forward/ reverse)	PCR amplification conditions	Cycles	Reference(s)
Internal transcribed spacer region of ribosomal DNA (ITS)	ITS5/ITS4	Initial denaturation: 94 °C – 5 min Denaturation: 94 °C – 45 sec Annealing: 52 °C – 1 min Extension: 72 °C – 90 sec Final extension: 72 °C – 10 min Hold: 4 °C – ∞	35	White et al. (1990)
		Initial denaturation: 94 °C – 5 min Denaturation: 94 °C – 45 sec
28S large subunit rDNA region (LSU)	LR0R/LR5	Annealing: 52 °C – 1 min Extension: 72 °C – 90 sec Final extension: 72 °C – 10 min Hold: 4 °C – ∞	35	Vilgalys and Hester (1990); Cubeta et al. (1991)
		Initial denaturation: 94 °C – 3 min Denaturation: 94 °C – 20 sec
Translation elongation factor 1-alpha (tef1-α)	EF1-728F/EF1-986R	Annealing: 56 °C – 1 min Extension: 72 °C – 90 sec Final extension: 72 °C – 10 min Hold: 4 °C – ∞	35	Carbone and Kohn (1999)
		Initial denaturation: 94 °C – 3 min Denaturation: 94 °C – 20 sec
Beta-tubulin (tub2)	Bt2a/Bt2b	Annealing: 52 °C – 1 min Extension: 72 °C – 90 sec Final extension: 72 °C – 10 min Hold: 4 °C – ∞	35	Glass and Donaldson (1995)