Distribution and evolution of the serine / aspartate racemase family in plants Author Uda, Kouji Laboratory of Biochemistry, Faculty of Science and Technology, Kochi University, Kochi, 780 - 8520, Japan Author Edashige, Yumika Author Nishimura, Rie Author Shikano, Yuuna Author Matsui, Tohru Author Radkov, Atanas D. Author Moe, Luke A. text Phytochemistry 2020 112164 2020-01-31 169 1 8 http://dx.doi.org/10.1016/j.phytochem.2019.112164 journal article 264478 10.1016/j.phytochem.2019.112164 43cf731e-852b-434a-a837-ab8cfefc7491 1873-3700 8293818

Table 2 Comparison of kinetic constants of plant AspRs and SerRs.

Species	Enzyme	Reaction	K m	k cat	k cat/ K m
			[mM]	[s −1]	[10-3 ·s −1 ·mM −1]
Manihot esculenta	AspR	L- Asp to D- Asp	3.94 ± 0.26	20.4 ± 0.9	5180 ± 110
		D- Asp to L- Asp	2.83 ± 0.15	15.7 ± 0.7	5540 ± 100
		l-Glu to D- Glu	131 ± 6	0.721 ± 0.0.043	5.47 ± 0.08
		D- Glu to L- Glu	181 ± 12	1.09 ± 0.11	6.01 ± 0.25
		L- Ser to D- Ser	172 ± 10	0.272 ± 0.019	1.59 ± 0.11
		D- Ser to L- Ser	307 ± 59	0.517 ± 0.090	1.69 ± 0.03
	SerR	L- Ser to D- Ser	9.06 ± 0.90	0.0600 ± 0.0077	6.76 ± 1.41
		D- Ser to L- Ser	8.23 ± 0.20	0.0517 ± 0.0051	6.28 ± 0.61
		L- Ser to Pyruvate	34.7 ± 1.8	0.203 ±.002	5.87 ± 0.29
		D- Ser to Pyruvate	43.5 ± 7.7	0.110 ± 0.007	2.63 ± 0.66
Solanum lycopersicum	AspR	L- Asp to D- Asp	2.23 ± 0.13	7.64 ± 0.49	3430 ± 170
		D- Asp to L- Asp	2.97 ± 0.37	7.56 ± 0.70	2570 ± 250
		L- Ser to D- Ser	64.2 ± 4.4	0.0631 ± 0.0037	0.986 ± 0.072
		D- Ser to L- Ser	242 ± 11	0.175 ± 0.023	0.721 ± 0.061
	SerR	L- Ser to D- Ser	11.7 ± 3.1	0.0287 ± 0.0049	2.51 ± 0.23
		D- Ser to L- Ser	23.3 ± 2.2	0.0428 ± 0.0038	1.84 ± 0.05
		L- Ser to Pyruvate	33.0 ± 1.8	0.343 ± 0.031	10.4 ± 0.1
		D- Ser to Pyruvate	42.1 ± 12.0	0.159 ± 0.025	3.89 ± 0.43
Sphagnum girgensohnii	AspR	L- Asp to D- Asp	9.57 ± 1.19	7.52 ± 0.61	790 ± 33
		D- Asp to L- Asp	4.43 ± 0.21	2.96 ± 0.12	667 ± 5
		L- Ser to D- Ser	32.1 ± 1.9	0.0599 ± 0.0037	1.86 ± 0.08
		D- Ser to L- Ser	106 ± 15	0.141 ± 0.021	1.32 ± 0.09
Spirogyra pratensis	AspR	L- Asp to D- Asp	4.00 ± 0.14	3.15 ± 0.23	790 ± 84
		D- Asp to L- Asp	8.47 ± 3.06	5.69 ± 1.49	694 ± 70
		L- Ser to D- Ser	15.8 ± 1.0	0.0641 ± 0.0047	4.06 ± 0.21
		D- Ser to L- Ser	22.8 ± 1.1	0.0773 ± 0.0085	3.38 ± 0.22