A taxonomic revision of Anthothela (Octocorallia: Scleraxonia: Anthothelidae) and related genera, with the addition of new taxa, using morphological and molecular data Author Moore, Kirrily M. Author Alderslade, Philip Author Miller, Karen J. text Zootaxa 2017 4304 1 1 212 journal article 32506 10.11646/zootaxa.4304.1.1 3b9bd2c4-7dee-46a3-a36d-80077b88a78b 1175-5326 842289 3D557C94-0783-4C39-80C3-9C321DA94800

TABLE 1. Primers used to amplify two mitochondrial gene regions (igr1– cox1 and mtMutS) including published external primers and newly designed internal primers. (adapted from McFadden et al. 2011). The last letter in each primer name indicates the primer direction (F = forward; R = reverse). Expected amplicon length and annealing temperatures are given for primer combinations.

Primer	Sequence 5’+3’	Fragment Size	T m°	Reference
COII (5’)-igr1- COI (5’): ~1000 bp
1) COII8068F	CCATAACAGGACTAGCAGCATC	~1000 (1&4) ~ 600 (1&2)	62.5	McFadden et al. 2004
2) PRANCOIR	CCTGTACCTGCCCCTTGTT		63.8	This study (igr1– cox1 A)
3) PRANCOIF	GGATTCGGAAATTGGTTTGT	~ 530 (3&4)	62.4	This study (igr1– cox1 B)
4) COIOCTR	ATCATAGCATAGACCATACC			France & Hoover 2002
mtMutS (5’): ~760 bp
5) ND42475F	TAGTTTTACTGGCCTCTAC	~990 (5&9) ~ 620(5&7)	50.9	Brugler et al. 2008
6) ND42599F	GCCATTATGGTTAACTATTAC	~870 (6&9) ~490 (6&7)	52.1	France & Hoover 2002
7) ANTHMSH1R	TCCGAACAGTCCTCTAAATTACAA			This study (mtMutS A)
8) ANTHMSH1F	GCTTCAAATGGGGTTTCCA	~490 (8&9)	64.3	This study (mtMutS B)
9) MUT3458R	TSGAGCAAAAGCCACTCC		61.6	Sánchez et al. 2003