Phylogenetic differentiation and taxonomic consequences in the Saurodactylus brosseti species complex (Squamata: Sphaerodactylidae), with description of four new species Author Javanmardi, Soheila Author Vogler, Sven Author Joger, Ulrich text Zootaxa 2019 2019-09-26 4674 4 401 425 journal article 25373 10.11646/zootaxa.4674.4.1 01473823-de33-48b2-8c73-3ab5b98f7e7b 1175-5326 3464970 F31BB0F8-76FA-4308-8708-227CC752EE37

TABLE 2. PCR conditions set for this study.

Gene	12S			ND4
Step	T (°C)	Duration	No. of Cycles	T (°C)	Duration	No. of Cycles
Initial Denaturation	94	90 sec.	1	96	3 min.	1
Denaturation	(94	45 sec.	35	(96	30 sec.	36
Annealing	52	45 sec.		52	45 sec.
Extension	72	60 sec.)		72	45 sec.)
Final Extension	72	10 min.	1	72	10 min.	1