Two remarkable new species of Glaucocharis (Lepidoptera, Crambidae, Crambinae) from the Ogasawara Islands, Japan Author Matsui, Yuki Entomological Laboratory, Faculty of Agriculture, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka, 819 - 0395, Japan. Author Hamaguchi, Junpei 0009-0006-3684-0214 Entomological Laboratory, Faculty of Agriculture, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka, 819 - 0395, Japan. hamaguchi.jumpei.281@s.kyushu-u.ac.jp Author Yagi, Sadahisa 0000-0002-4261-1219 Insect DX Laboratory, Faculty of Agriculture, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka, 819 - 0395, Japan. yagi.sadahisa@gmail.com Author Hirowatari, Toshiya 0000-0002-6839-2229 Entomological Laboratory, Faculty of Agriculture, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka, 819 - 0395, Japan. & Entomological Laboratory, Faculty of Agriculture, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka, 819 - 0395, Japan. irowat_t@agr.kyushu-u.ac.jp text Zootaxa 2024 2024-12-02 5543 1 83 96 https://doi.org/10.11646/zootaxa.5543.1.4 journal article 306541 10.11646/zootaxa.5543.1.4 4e8825bb-65d4-49fb-a52c-5da5b28c4a03 1175-5326 14385137 DA37B0E4-61F6-4C12-958D-91E15DC76B4C
TABLE 2. List of PCR primers used.
GenePrimerDirectionSequence (5' -> 3')Annealing temperatureReference
COITY-J-1460- SpiloForwardTACAATTTATCGCTTAATACTCAGCC55Matsui et al. 2021
COITL2-N-3014- SpiloReverseTCCATTACATATAATCTGCCATATTA55Matsui et al. 2021
CADCAD743fForwardGGNGTNACNACNGCNTGYTTYGARCC56Wahlberg & Wheat 2008
CADCAD1028rReverseTTRTTNGGNARYTGNCCNCCCAT56Wahlberg & Wheat 2008
MDHMDHfForwardGAYATNGCNCCNATGATGGGNGT58Wahlberg & Wheat 2008
MDHMDHrReverseAGNCCYTCNACDATYTTCCAYTT58Wahlberg & Wheat 2008
RpS5RpS5fForwardATGGCNGARGARAAYTGGAAYGA62Wahlberg & Wheat 2008
RpS5RpS5rReverseCGGTTRGAYTTRGCAACACG62Wahlberg & Wheat 2008