Morphology and multigene phylogeny reveal a novel Stagonospora species (Massarinaceae, Dothideomycetes) from Thailand Author Bhagya, Amuhenage T. 0009-0002-3988-298X School of Science, Mae Fah Luang University, Chiang Rai 57100, Thailand & Center of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai 57100, Thailand bhagyatrex@gmail.com Author Phukhamsakda, Chayanard 0000-0002-1033-937X Center of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai 57100, Thailand chayanard.phu@mfu.ac.th Author Tanaka, Kazuaki 0000-0002-7037-0774 Faculty of Agriculture and Life Science, Hirosaki University, 3 Bunkyo-cho, Hirosaki, Aomori 036 - 8561, Japan k-tanaka@hirosaki-u.ac.jp Author Jones, E. B. Gareth 0000-0002-7286-5471 Department of Botany and Microbiology, College of Science, King Saud University, P. O Box 2455, Riyadh, 11451, Kingdom of Saudi Arabia torperadgj@gmail.com text Phytotaxa 2024 2024-04-17 644 4 281 293 http://dx.doi.org/10.11646/phytotaxa.644.4.4 journal article 301719 10.11646/phytotaxa.644.4.4 d4d31939-aadd-48ee-be34-c59055554264 1179-3163 13214798

TABLE 1. The amplified loci, primers, and PCR thermal cycle protocols were used in this study.

Genes/loci	PCR primers (forward/reverse) PCR conditions		Reference
LSU	LR0R/LR5	95 °C; 5 min (94 °C; 30 s, 55 °C; 50 s, 72 °C; 90 s) × 35 thermal cycles, 72 °C; 10 min	Vilgalys & Hester (1990)
ITS SSU	ITS1 / ITS4 NS1 / NS4	94 °C; 2 min (95 °C; 30 s, 55 °C; 50 s, 72 °C; 90 s) × 35 thermal cycles, 72 °C; 10 min	White et al. (1990)
tef 1-α	EF1-983F / EF1-2218R	95 °C; 5 min (95 °C; 30 s, 58 °C; 50 s, 72 °C; 1 min) Rehner & Buckley (2005) × 35 thermal cycles, 72 °C; 10 min