The good, the bad and the boa: An unexpected new species of a true boa revealed by morphological and molecular evidence Author Gonzalez, Rodrigo Castellari Author Lima, Lorena Corina Bezerra de Laboratório de Ecologia e Evolução, Instituto Butantan, São Paulo, Brazil Author Passos, Paulo Author Silva, Maria José J. Laboratório de Ecologia e Evolução, Instituto Butantan, São Paulo, Brazil text PLoS ONE 2024 e 0298159 2024-04-17 19 4 1 29 journal article 306483 10.1371/journal.pone.0298159 75511d24-63da-45a7-8a1e-e497951ccd10 1932-6203 PMC11023597 38630841 13151904

Table 1. Samples used in the present work for which unpublished sequences were produced, containing morphotype, voucher or collection number in the field, locality, state (available only for Brazil), country of origin, and geographical coordinates.

Sample	Taxa	Voucher	Locality	FU	Country	Latitude	Longitude	cyt b	ND4	NTF3	ODC	Map Number
(Continued)
IBSP = Instituto Butantan (São Paulo, SP, Brazil); CHUNB = Coleção Herpetológica—Universidade de Brasília (Brasília, Brazil); CHBEZ = Coleção Herpetológica do Departamento de Botânica, Ecologia e Zoologia—UFRN (Natal, Rio Grande do Norte, Brazil); CORBIDI = Centro de Ornitologia e Biodiversidad (Lima, Peru); FIT = Faculdades Integradas do Tapajós (Santarém, PA, Brazil); MBIB = Museu Biológico—Instituto Butantan (São Paulo, SP, Brazil); MNRJ = Museu Nacional (Rio de Janeiro, RJ, Brazil); MPEG = Museu Paraense Emílio Goeldi (Belém, PA, Brazil); QCZAR = Museo de Zoología, Pontificia Universidad Católica del Ecuador; UVM = Universidade de Medicina Veterinária (Viena, Áustria). MJJS = sample from Laboratório de Ecologia e Evolução, LEEV. LCBL were collected by Lorena Corina Bezerra de Lima; IBSP83333, LCBL21, LCBL23 from Instituto Butantan and Criadouro Jibóias Brazil were identified as B. occidentalis. ALT-256, RVSS-33, UFA 223: Donated by Dr. Felipe Franco Curcio, UFMT * Approximate coordinates. Genes amplified for each sample: cyt- b = cytochrome b; ND4 = NADH dehydrogenase subunit 4; NTF3 = neurotrophin 3; ODC = ornithine decarboxylase. The last column refers to the location number from where the samples were collected (which correspond to same number in the map of Fig 1). See material and methods for the rationale of identifications to new sequences based on voucher specimens. The paratypes of the new species are marked in bold.
1	B. c. constrictor	LCBL1	Rio Branco	AC	Brazil	-9.9560	-67.8670	x	x	x	x	1
2	B. c. constrictor	LCBL2	Senador Guiomard	AC	Brazil	-10.0696	-67.6091	x	x	x	x	2
3	B. c. constrictor	LCBL12	Manaus	AM	Brazil	-3.0038	-59.9187	x				3
4	B. c. constrictor	LCBL13	Manaus	AM	Brazil	-3.0038	-59.9187	x	x	x	x	4
5	B. c. constrictor	CHUNB53038	Alta Floresta d’Oeste	RO	Brazil	-11.9287	-61.9829	x				5
6	B. c. constrictor	H1293	Porto Velho	RO	Brazil	-8.7649	-63.8683	x	x	x	x	6
7	B. c. constrictor	CHUNB22028	Guajará-Mirim	RO	Brazil	-10.7741	-65.3368	x	x	x	x	7
8	B. c. constrictor	HJ0547	Mutum-Paraná	RO	Brazil	-9.2868	-64.5488	x				8
9	B. c. constrictor	HJ0203	UHE Jirau	RO	Brazil	-9.2582	-64.6498	x	x	x	x	9
10	B. c. constrictor	RVSS33	Nova Ubiratã	MT	Brazil	-13.0357	-55.2599	x	x	x	x	10
11	B. c. constrictor	UFA223	Paranaíta	MT	Brazil	-9.6731	-56.4714	x	x	x	x	11
12	B. c. constrictor	ALT256	Alta Floresta	MT	Brazil	-9.8680	-56.0805	x				12
13	B. c. constrictor	CTMZ05754	Guiratinga	MT	Brazil	-16.3463	-53.7561	x	x	x	x	13
14	B. c. constrictor	CTMZ06126	Itaúba	MT	Brazil	-11.0080	-55.2427	x	x	x	x	14
15	B. c. constrictor	MPEG24581	Belém	PA	Brazil	-1.4436	-48.4410	x	x	x	x	15
16	B. c. constrictor	MPEG21584	Melgaço	PA	Brazil	-1.7977	-50.7385	x	x	x	x	16
17	B. c. constrictor	MNRJ16818	Porto Trombetas	PA	Brazil	-1.4549	-56.3818	x	x	x	x	17
18	B. c. constrictor	FIT5	Santarém	PA	Brazil	-2.4406	-54.7349	x				18
19	B. c. constrictor	FIT8	Santarém	PA	Brazil	-2.4406	-54.7349	x	x	x	x	19
20	B. c. constrictor	BM326	Vitória do Xingu	PA	Brazil	-3.1277	-51.7003	x				20
21	B. c. constrictor	LCBL20	Xambioá	TO	Brazil	-6.4130	-48.5351	x	x	x	x	21
22	B. c. constrictor	QCZAR6476	Orellana (província)		Equador	-6.5826	-77.1986	x				22
23	B. c. constrictor	MNRJ	Falcón (State)		Venezuela	11.1085	-6.9784	x				23
24	B. atlantica sp. nov.	CHBEZ42	Rio Largo	AL	Brazil	-9.4819	-35.8394	x	x	x	x	24
25	B. atlantica sp. nov.	IBSP79031	Salvador	BA	Brazil	-12.9731	-38.4871	x	x	x	x	25
26	B. atlantica sp. nov.	IBSP79063	Cachoeiro do Itapemirim	ES	Brazil	-20.8616	-41.1285	x	x	x	x	26
27	B. atlantica sp. nov.	MNRJ22936	Cabo Frio	RJ	Brazil	-22.9218	-42.0412	x	x	x	x	27
28	B. atlantica sp. nov.	MNRJ19564	Rio de Janeiro	RJ	Brazil	-22.9784	-43.3050	x				28
29	B. atlantica sp. nov.	MNRJ19740	Rio de Janeiro	RJ	Brazil	-22.9784	-43.3050	x	x	x	x	29
30	B. atlantica sp. nov.	MNRJ20700	Teresópolis	RJ	Brazil	-22.4161	-42.9812	x				30
31	B. atlantica sp. nov.	CHBEZ1196	Caicó	RN	Brazil	-6.4669	-37.0856	x	x	x	x	31
32	B. atlantica sp. nov.	CHBEZ1254	Serra Negra do Norte	RN	Brazil	-6.6624	-37.3961	x	x	x	x	32
33	B. c. amarali	CTMZ06043	Barra do Garças	MT	Brazil	-15.8534	-52.2704	x	x	x	x	33
34	B. c. amarali	CTMZ00603	Paulínia	SP	Brazil	-22.7650	-47.1489	x	x	x	x	34
35	B. c. amarali	IBSP79064	Esmeraldas	MG	Brazil	-19.8072	-44.1800	x	x	x	x	35
36	B. c. amarali	IBSP84579	Fortaleza de Minas	MG	Brazil	-20.8484	-46.7085	x	x	x	x	36
37	B. c. amarali	IBSP84578	Cabreúva	SP	Brazil	-23.3051	-47.1362	x	x	x	x	37
38	B. c. amarali	IBSP84577	Guarulhos	SP	Brazil	-23.4259	-46.5376	x	x	x	x	38
39	B. c. amarali	IBSP84595	Itú	SP	Brazil	-23.2653	-47.2870	x	x	x	x	39
40	B. c. amarali	MPEG22195	Canaã dos Carajás	PA	Brazil	-6.5300	-49.8532	x	x	x	x	40
41	B. c. amarali	MJJS270	Paraúna	GO	Brazil	-16.9680	-58.3416	x				41
42	B. c. amarali	ESTR00015	Carolina	MA	Brazil	-7.3354	-47.4640	x				42
43	B. c. amarali	CHUNB44537	Buritizeiro	MG	Brazil	-17.3598	-44.9731	x	x	x	x	43
44	B. c. amarali	MNRJ20989	Corumbá	MS	Brazil	-19.0079	-57.6519	x	x	x	x	44
45	B. i. imperator	QCZAR5636	El Mango (região)	–	Equador	-2.45232	-79.62156	x				45
46	* B. occidentalis	IBSP83333	–	–	Argentina and Paraguay	–	–	x	x	x	x	46
47	* B. occidentalis	LCBL21	–	–	Argentina and Paraguay	–	–	x	x	x	x	47
48	* B. occidentalis	LCBL23	–	–	Argentina and Paraguay	–	–	x	x	x	x	48