Detecting the effect of ACCase-targeting herbicides on ACCase activity utilizing a malachite green colorimetric functional assay Author Basak, Suma Graduate Research Assistant, Department of Crop, Soil, and Environmental Sciences, Auburn University, Auburn, AL, USA; Author Alam, Md. Jahangir Graduate Research Assistant, Department of Chemistry and Biochemistry, Auburn University, Auburn, AL, USA; Author Goodwin, Douglas Professor, Department of Chemistry and Biochemistry, Auburn University, Auburn, AL, USA; Author Harris, James Research Technician, Department of Crop, Soil, and Environmental Sciences, Auburn University, Auburn, AL, USA; Author Patel, Jinesh D. Research Associate, Department of Crop, Soil, and Environmental Sciences, Auburn University, Auburn, AL, USA; Author McCullough, Patrick Associate Professor, Department of Crop and Soil Sciences, University of Georgia, Griffin, GA, USA and Author McElroy, J. Scott Professor, Department of Crop, Soil, and Environmental Sciences, Auburn University, Auburn, AL, USA text Weed Science 2022 Cambridge, England 2021-08-11 70 1 14 19 http://dx.doi.org/10.1017/wsc.2021.57 journal article 10.1017/wsc.2021.57 1550-2759 11553328

non-treated the to relative concentration herbicide ACCase-targeting increasing to activity) ACCase (carboxylase A acetyl-coenzyme of percent for biotypes ciliaris Digitaria susceptible model fit and least-squares resistant the of Comparison with. measured 1 control Table

Herbicide a	Biotypeb	Equationc	Parameter estimates and confidence intervalsd
		Y = bottom þ (top − bottom) / (1 þ 10 ^ (X − logIC 50))	Bottom	95 % CI	Top	95 % CI	Log	95 % CI
							IC 50
Sethoxydim	R 1	Y = − 38.3 þ (99.5 þ 38.3) /(1 þ 10 ^ (X − 1.2))	− 38.3	(− 42.4, − 34.2)	99.5	(98.6, 100.5)	1.1	(1.1, 1.2)
	R2	Y = − 103.5 þ (99.7 þ 103.5) / (1 þ 10^ (X − 1.6))	− 103.5	(− 117.7, − 89.3)	99.7	(98.8, 100.6)	1.6	(1.6, 1.7)
	S	Y = − 3.3 þ (114.2 þ 3.3) / (1 þ 10 ^ (X þ 0.2))	3.3	(1.4, 5.2)	114.2	(109.4,118.9)	− 0.1	(− 0.2, − 0.1)
Clethodim	R 1	Y = − 6.9 þ (102.1 þ 6.9) / (1 þ 10 ^ (X − 0.5))	− 6.9	(− 9.0, − 4.8)	102.1	(100.3, 103.9)	0.5	(0.5, 0.6)
	R2	Y = − 17.1 þ (99.1 þ 17.1) / (1 þ 10 ^ (X − 0.9))	− 17.1	(− 21.2, − 13)	99.1	(97.2, 101.1)	0.9	(0.8, 0.9)
	S	Y = 4.2 þ (120.1 − 4.2) / (1 þ 10 ^ (X þ 0.3))	4.2	(2.3, 6.1)	120.1	(114.2, 125.9)	− 0.3	(− 0.4, − 0.3)
Fluazifop- p - butyl	R 1	Y = − 20.4 þ (99.4 þ 20.4) /(1 þ 10 ^ (X − 0.9))	− 20.4	(− 24.5, − 16.4)	99.4	(97.7, 101)	0.9	(0.9, 1.0)
	R2	Y = − 39.8 þ (98.2 þ 39.8) /(1 þ 10 ^ (X − 1.2))	− 39.8	(− 47.4, − 32.3)	98.2	(96.7, 99.8)	1.2	(1.1, 1.3)
	S	Y = − 0.4 þ (119.5 þ 0.4) / (1 þ 10 ^ (X þ 0.3))	− 0.4	(− 1.2, 0.4)	119.5	(117.3, 121.8)	− 0.2	(− 0.3, − 0.3)
Pinoxaden	R 1	Y = − 30.2 þ (97.8 þ 30.2) /(1 þ 10 ^ (X − 1.1))	− 30.2	(− 36.8, − 23.6)	97.8	(95.9, 99.7)	1.1	(1.0, 1.2)
	R2	Y = − 67.7 þ (99.1 þ 67.7) /(1 þ 10 ^ (X − 1.5))	− 67.7	(− 84.5, − 50.8)	99.1	(97.3, 100.9)	1.5	(1.4, 1.5)
	S	Y = − 2.1 þ (106.1 þ 2.1) / (1 þ 10 ^ (X − 0.2))	− 2.1	(− 3.4, − 0.8)	106.1	(104.1, 108)	0.2	(0.1, 0.2)