Detection and characterization of diverse coccidian protozoa shed by California sea lions Author Girard, Yvette A. Karen C. Drayer Wildlife Health Center, School of Veterinary Medicine, University of California, Davis, CA, USA Author Johnson, Christine K. Karen C. Drayer Wildlife Health Center, School of Veterinary Medicine, University of California, Davis, CA, USA Author Fritz, Heather M. Department of Pathology, Microbiology and Immunology, School of Veterinary Medicine, University of California, Davis, CA, USA & Department of Veterinary Pathology and Microbiology, College of Veterinary Medicine, Washington State University, Pullman, WA, USA Author Shapiro, Karen Department of Pathology, Microbiology and Immunology, School of Veterinary Medicine, University of California, Davis, CA, USA Author Packham, Andrea E. Department of Pathology, Microbiology and Immunology, School of Veterinary Medicine, University of California, Davis, CA, USA Author Melli, Ann C. Department of Pathology, Microbiology and Immunology, School of Veterinary Medicine, University of California, Davis, CA, USA Author Carlson-Bremer, Daphne Vanderbilt Institute for Global Health, Vanderbilt University, Nashville, TN, USA Author Gulland, Frances M. The Marine Mammal Center, Sausalito, CA, USA Author Rejmanek, Daniel California Animal Health and Food Safety, Davis, CA, USA Author Conrad, Patricia A. Karen C. Drayer Wildlife Health Center, School of Veterinary Medicine, University of California, Davis, CA, USA & Department of Pathology, Microbiology and Immunology, School of Veterinary Medicine, University of California, Davis, CA, USA text International Journal for Parasitology: Parasites and Wildlife 2016 2016-04-30 5 1 5 16 http://dx.doi.org/10.1016/j.ijppaw.2015.11.003 journal article 292058 10.1016/j.ijppaw.2015.11.003 0497e66e-da65-45e4-829b-be252b0a9965 2213-2244 PMC4840268 27141438 10668346

Table 3 Prevalence and genetic diversity of coccidian species identified by microscopy in fecal samples of free-ranging California sea lions at coastal haul-out sites.

Location (Sample identifier)	No. samples examined	Time period	No. oocyst-positive samples	Sample ID (Coccidian species)
a Oocysts were identified by microscopy during isolation, but DNA was insufficient for molecular characterization. b The amplified ITS-1 region of ̃Ano11 is genetically distinct from other coccidian isolates (See Fig. 1). c The presence of Sarcocystis neurona -like DNA was confirmed by PCR and sequence analysis. Sporocysts were not identified in fecal samples by microscopy.
	for oocysts		(Prevalence)
White Rock (WR-)	94	July 2011 ‾ August 2012	3 (3.2%)	WR101 (Coccidia A)
				WR111 (uncharacterized) a
				WR121 (Coccidia A)
Ano ̃Nuevo (̃Ano-)	48	May 2011 ‾ October 2011	4 (8.3%)	̃Ano11 (novel)b
				Ãno44 (Coccidia B)
				Ãno50 (Coccidia B)
				Ãno53 (Coccidia A)
Point Lobos (PL-)	70	August 2011 ‾ Sept 2012	6 (8.6%)	PL11 (Coccidia B + S. neurona -like c)
				PL 27 (Coccidia A + S. neurona -like c)
				PL50 (Coccidia A)
				PL58 (Coccidia B)
				PL68 (Coccidia A)
				PL75 (Coccidia B)
Total	244		13 (5.3%)	6 Coccidia A
				5 Coccidia B
				2 S. neurona -like
				1 uncharacterized a
				1 novel b