Two new species of stone loaches of the genus Barbatula (Cypriniformes: Nemacheilidae) from Europe with a neotype designation of B. barbatula (Teleostei: Nemacheilidae) Author Calegari, Bárbara B. Aquatic Ecology and Evolution, Institute of Ecology and Evolution, University of Bern, Bern, Switzerland & Department of Fish Ecology and Evolution, EAWAG, Swiss Federal Institute for Aquatic Science and Technology, Kastanienbaum, Switzerland & Wyss Academy for Nature at the University of Bern, Bern, Switzerland barbara.calegari@unibe.ch Author Freyhof, Jörg Museum für Naturkunde, Leibniz Institute for Evolution and Biodiversity Science, Berlin, Germany Author Waldock, Conor Aquatic Ecology and Evolution, Institute of Ecology and Evolution, University of Bern, Bern, Switzerland & Department of Fish Ecology and Evolution, EAWAG, Swiss Federal Institute for Aquatic Science and Technology, Kastanienbaum, Switzerland & Wyss Academy for Nature at the University of Bern, Bern, Switzerland Author Wegscheider, Bernhard Aquatic Ecology and Evolution, Institute of Ecology and Evolution, University of Bern, Bern, Switzerland & Department of Fish Ecology and Evolution, EAWAG, Swiss Federal Institute for Aquatic Science and Technology, Kastanienbaum, Switzerland & Wyss Academy for Nature at the University of Bern, Bern, Switzerland Author Josi, Dario Aquatic Ecology and Evolution, Institute of Ecology and Evolution, University of Bern, Bern, Switzerland & Department of Fish Ecology and Evolution, EAWAG, Swiss Federal Institute for Aquatic Science and Technology, Kastanienbaum, Switzerland & Wyss Academy for Nature at the University of Bern, Bern, Switzerland Author Rüber, Lukas Aquatic Ecology and Evolution, Institute of Ecology and Evolution, University of Bern, Bern, Switzerland & Naturhistorisches Museum Bern, Bern, Switzerland Author Seehausen, Ole Aquatic Ecology and Evolution, Institute of Ecology and Evolution, University of Bern, Bern, Switzerland & Department of Fish Ecology and Evolution, EAWAG, Swiss Federal Institute for Aquatic Science and Technology, Kastanienbaum, Switzerland text Journal of Fish Biology 2025 2025-07-02 107 4 1364 1397 journal article 10.1111/jfb.70108 272faa11-1c7b-4955-8172-7f1e74f6b939 1095-8649 17584041

TABLE 4 Pairwise genetic distance (%) of Barbatula species from Western Europe clade based on coI gene.

Species		%
B. barbatula	B. barbatula (n = 6)	0.00
	B. fluvicola B. hispanica B. leoparda B. ommata Barbatula sp. (W4)	2.71 – 3.34 1.38 – 1.57 2.46 – 2.61 2.15 – 2.61 1.69 – 2.00
B. fluvicola	B. fluvicola (n = 101)	0.00 – 0.80
	B. hispanica B. leoparda B. ommata Barbatula sp. (W4)	3.03 – 3.98 3.38 – 4.46 2.39 – 3.31 3.41 – 4.70
B. hispanica	B. hispanica (n = 42)	0.00 – 0.32
	B. leoparda B. ommata Barbatula sp. (W4)	1.73 – 2.34 2.46 – 3.28 1.09 – 1.96
B. leoparda	B. leoparda (n = 2)	0.77
	B. ommata Barbatula sp. (W4)	3.07 – 3.69 2.11 – 3.02
B. ommata	B. ommata (n = 52)	0.00 – 0.92
	Barbatula sp. (W4)	2.77 – 3.91
Barbatula sp. (W4)	Barbatula sp. (W4) (n = 19)	0.00 – 1.68